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mouse anti human icam  (R&D Systems)


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    Structured Review

    R&D Systems mouse anti human icam
    Mouse Anti Human Icam, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+icam+1+antibody/Human+ICAM-1%2FCD54+Antibody/pm41944190-237-44-47
    Average 93 stars, based on 110 article reviews
    mouse anti human icam - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: Human microvascular pericyte basement membrane remodeling regulates neutrophil recruitment.
    Article Snippet: Objective: Neutrophil extravasation at post-capillary venules, consisting of EC, PC, and the shared ECM, increases following fibrotic remodeling in the lung, liver, and skin.. The role of fibrotic pericyte-derived ECM in regulating EC activation and neutrophil recruitment remains unexplored.. Methods: To elucidate the role of human pericyte-derived ECM in EC activation, we characterized PC-derived ECM following transforming growth factor-b1, IL-1b, CCL2, or bleomycin activation, and examined surface adhesion molecule expression and neutrophil recruitment by EC cultured on PC-ECM.

    Labeling:

    Article Title: Human microvascular pericyte basement membrane remodeling regulates neutrophil recruitment.
    Article Snippet: Objective: Neutrophil extravasation at post-capillary venules, consisting of EC, PC, and the shared ECM, increases following fibrotic remodeling in the lung, liver, and skin.. The role of fibrotic pericyte-derived ECM in regulating EC activation and neutrophil recruitment remains unexplored.. Methods: To elucidate the role of human pericyte-derived ECM in EC activation, we characterized PC-derived ECM following transforming growth factor-b1, IL-1b, CCL2, or bleomycin activation, and examined surface adhesion molecule expression and neutrophil recruitment by EC cultured on PC-ECM.

    Single Cell:

    Article Title: Intercellular adhesion molecule-1 on synovial cells attenuated interleukin-6-induced inhibition of osteoclastogenesis induced by receptor activator for nuclear factor ?B ligand
    Article Snippet: .. After culturing, cells were treated with trypsin-ethylenediamine tetraacetic acid (EDTA) (G ibco , Grand Island, NY, USA) to obtain a single cell suspension, and cells were then stained with mouse anti-human ICAM-1 antibody (R&D Systems) and Alexa Fluor 488 conjugated anti-mouse IgG (Molecular Probes, Eugene, OR, USA). .. After staining, ICAM-1 expression on the cell surface was measured with a fluorescence-activated cell sorter (FACSCanto II; BD Biosciences, Franklin Lakes, NJ, USA), using FACSDiva software (BD Biosciences) for data acquisition and FlowJo software (Tree Star, Ashland, OR, USA) for data analysis.

    Suspension:

    Article Title: Intercellular adhesion molecule-1 on synovial cells attenuated interleukin-6-induced inhibition of osteoclastogenesis induced by receptor activator for nuclear factor ?B ligand
    Article Snippet: .. After culturing, cells were treated with trypsin-ethylenediamine tetraacetic acid (EDTA) (G ibco , Grand Island, NY, USA) to obtain a single cell suspension, and cells were then stained with mouse anti-human ICAM-1 antibody (R&D Systems) and Alexa Fluor 488 conjugated anti-mouse IgG (Molecular Probes, Eugene, OR, USA). .. After staining, ICAM-1 expression on the cell surface was measured with a fluorescence-activated cell sorter (FACSCanto II; BD Biosciences, Franklin Lakes, NJ, USA), using FACSDiva software (BD Biosciences) for data acquisition and FlowJo software (Tree Star, Ashland, OR, USA) for data analysis.

    Staining:

    Article Title: Intercellular adhesion molecule-1 on synovial cells attenuated interleukin-6-induced inhibition of osteoclastogenesis induced by receptor activator for nuclear factor ?B ligand
    Article Snippet: .. After culturing, cells were treated with trypsin-ethylenediamine tetraacetic acid (EDTA) (G ibco , Grand Island, NY, USA) to obtain a single cell suspension, and cells were then stained with mouse anti-human ICAM-1 antibody (R&D Systems) and Alexa Fluor 488 conjugated anti-mouse IgG (Molecular Probes, Eugene, OR, USA). .. After staining, ICAM-1 expression on the cell surface was measured with a fluorescence-activated cell sorter (FACSCanto II; BD Biosciences, Franklin Lakes, NJ, USA), using FACSDiva software (BD Biosciences) for data acquisition and FlowJo software (Tree Star, Ashland, OR, USA) for data analysis.

    Electron Microscopy:

    Article Title: Microparticles From Human Atherosclerotic Plaques Promote Endothelial ICAM-1–Dependent Monocyte Adhesion and Transendothelial Migration
    Article Snippet: After 24 hours, transmigrated cells in the lower chamber were labeled with anti-human LFA1-phycoerythrin (20 μL/test) antibodies or their corresponding isotype-matched IgG controls (BD Pharmingen; San Diego, CA) and numbered by flow cytometry analysis (FACSCanto II flow cytometer; BD Biosciences, San Jose, CA) by comparison to Flowcount Calibrator beads of known concentration. .. Immunofluorescent analysis and scanning electron microscopy In order to test the hypothesis of a transfer of ICAM-1 from MPs to endothelial cells, plaque MPs were incubated for 1 hour with a primary mouse anti-human ICAM-1 antibody (10 μg/mL; R&D Systems; Minneapolis, MN). ..

    Incubation:

    Article Title: Microparticles From Human Atherosclerotic Plaques Promote Endothelial ICAM-1–Dependent Monocyte Adhesion and Transendothelial Migration
    Article Snippet: After 24 hours, transmigrated cells in the lower chamber were labeled with anti-human LFA1-phycoerythrin (20 μL/test) antibodies or their corresponding isotype-matched IgG controls (BD Pharmingen; San Diego, CA) and numbered by flow cytometry analysis (FACSCanto II flow cytometer; BD Biosciences, San Jose, CA) by comparison to Flowcount Calibrator beads of known concentration. .. Immunofluorescent analysis and scanning electron microscopy In order to test the hypothesis of a transfer of ICAM-1 from MPs to endothelial cells, plaque MPs were incubated for 1 hour with a primary mouse anti-human ICAM-1 antibody (10 μg/mL; R&D Systems; Minneapolis, MN). ..

    Binding Assay:

    Article Title: Interleukin-1β induces hyaluronan and CD44-dependent cell protrusions that facilitate fibroblast-monocyte binding.
    Article Snippet: .. The followingprimary antibodieswere used: rat anti-human CD44 antibody (Calbiochem A020), biotinylated hyaluronic acid binding protein (bHABP) (Seikagaku Corporation, Tokyo, Japan), mouse anti-human ICAM-1 antibody (R&D Systems, Abingdon, UK), rat anti-CD44 FITC conjugate (Abcam), and cholera toxin subunit B (CtxB) Alexa Fluor 594 conjugate (Molecular Probes, Paisley, UK, and Invitrogen). .. The following secondary antibodies were used: rabbit anti-rat tetramethyl rhodamine isothiocyanate, goat anti-mouse FITC (Dakocytomation, Cambridgeshire, YK), and Avidin-FITC (Vector Laboratories, Burlingame, CA).

    Control:

    Article Title: Mechanosensation by endothelial PIEZO1 is required for leukocyte diapedesis.
    Article Snippet: .. For antibody-mediated clustering of ICAM-1, sheep anti-mouse IgG-coupled dynabeads (M280; Invitrogen) were coated with mouse anti-human ICAM-1 antibody (BBIG-I1; R&D Systems) or immunoglobulin IgG1 (IgG1) control (MAB002; R&D Systems) overnight at 4 C according to the manufacturer’s protocol. ..

    Article Title: Mechanosensation by endothelial PIEZO1 is required for leukocyte diapedesis
    Article Snippet: .. For antibody-mediated clustering of ICAM-1, sheep anti-mouse IgG-coupled dynabeads (M280; Invitrogen) were coated with mouse anti-human ICAM-1 antibody (BBIG-I1; R&D Systems) or immunoglobulin IgG1 (IgG1) control (MAB002; R&D Systems) overnight at 4°C according to the manufacturer’s protocol. ..



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